IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/19

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Date: 09/19/13 People in lab: Levi Palmer

Title: Gel Electrophoresis of 9/14 PCR of nosZ norCB

Start Time: 1:30 PM

Purpose: To check for nosZ and norCB

Protocol: LTM ed. 2

Well 1 2 3 4 5 6 7 8
Sample nosZ Ladder norCB

Results: Possible norCB product, very weak band

Stop Time: 3:20 PM

Date: 09/19/13 People in lab: Emily Puleo, Kelsey Crossen

Title: Plating DH5alpha for seed plate

Start Time: 6:40 PM

Purpose: To grow a seed plate of DH5alpha to make competent cells with

Protocol: LTM ed. 2

Products:

Sample Label Description
9/16/13 comp cells comp cell seed stock from -80C freezer

Stop Time: 7:00 PM

Next: Inoculation



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