IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/26

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Date: 7/26/14 People in lab: Emily Puleo

Title: Miniprep and nanodrop Start Time: 9:30 am Purpose: Purification of plasmids that most probably have hmp and testing of quality of such Protocol: LTM Ed. 2 Miniprep and nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
7/26 MP 1 ng/uL = 30.6; 260/280 = 1.97; 260/230 = 2.33 7/25 I 1 1
7/26 MP 2 ng/uL = 29.9; 260/280 = 1.87; 260/230 = 2.4 7/25 I 2 1
7/26 MP 3 ng/uL = 34.4; 260/280 = 1.9; 260/230 = 2.35 7/25 I 3 1



Results: Notes: Forgot to mix MP1 and MP2 so retested Stop Time: 11:30 am Next: digest with EcoRI to confirm presence of hmp

Date: 7/26/14 People in lab: Emily Puleo

Title: Digestion Start Time: 11:40 am Purpose: To check for the presence of hmp Protocol: LTM Ed. 2 Digestion Exceptions: Products:

Sample Label Description Source Label Quantity
7/26 D 1 hmp digested with EcoRI 7/26 MP 1 1
7/26 D 2 hmp digested with EcoRI 7/26 MP 2 1
7/26 D 3 hmp digested with EcoRI 7/26 MP 3 1



Results: Notes: Stop Time: 1:35 pm Next: Run a gel to determine size

Date: 7/26/14 People in lab: Emily Puleo

Title: Gel Electrophoresis Start Time: 1:45 pm Purpose: To check product size from digestion Protocol: LTM Ed. 2 Gels Exceptions: Products:

Well 1 2 3 4 5 6 7 8
- - - D1 D2 Ladder D3 -

Products: Results: Notes: Stop Time: 2:30 pm Next:



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