IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/22/Shan Shen
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10:00
There are 3 clones on the SupD + terminator plate.
PCR to test whether the clones are correct.
| Total | 10μL |
| Template | Clones |
| dNTP | 2μL |
| Buffer | 1μL |
| Taq | 0.5μL |
| For-primer | 0.5μL |
| Rev-primer | 0.5μL |
| ddH2O | 5.5μL |
14:00
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, negative control, colony 1, colony 2, colony 3.
Results:
Results:
It is difficult to say which colony is correct.
22:30
Miniprep SupD + terminator colonies 1,2,3.
| Number of the plasmids | Concentration(ng/μL) |
| SupD1+1-23L | 140 |
| SupD2+1-23L | 110 |
| SupD3+1-23L | 165 |
