IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/13/Shan Shen
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Electrophoresis to test the digestion products of 1-18A
The order and the amount of the samples: marker 5μL, plasmids of 1-18A 2μL, digestion products of 1-18A 20μL.
Results:
The digestion products still run slower than the uncut plasmids, but 1-18A is tested to be gel-bad according to the registry, so that the products are probably correct.
10:30
Continue to prepare competent cells.
13:50
Ligation of the 1-18A vector and 1-12O (GFP) inserts.
| Total | 10μL |
| Vectors | 2μL |
| Inserts | 6μL |
| T4 ligase | 1μL |
| Buffer | 1μL |
22:30
Transformation of the ligation products and 1-1D, 1-6I to test the efficiency of the competent cells.
