IGEM:Paris Bettencourt 2012/Protocols/Minimal Medium

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Minimal Medium

  • 250 ml A30 (30% Agar auto claved)
  • 100 ml 5x M9 Salts
    • PO4
    • NH4
    • NaCl
  • Complete 500mL with Water
  • 20µl of 1M MgSO4
  • 100µl 1M CaCl2 (slowly)
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