IGEM:Peking/2007/Switch-Notebook/2007-8-21
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precipitation of plx012
colony PCR to test lacI-pcc009(old)
no one seems to be right!
re-ligation of pcc009 and lacIq
transformation tonight
re-PCR lacIq
re-PCR ss1, rs1, rs2, rd2, rd3, cd1
from left to right is CD1, SS1, RD2. RD3, RD3, RS1, RS2
lacI double digestion
lacI 10uL buffer H 10uL XhoI 2uL SalI 2uL ddH2O 76uL total 100uL
pcc009 digestion
XhoI and SalI
the left one is pcc009-1, 0.25uL enzyme/20uL the right one is pcc009-3, 2uL enzyme/100uL
