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plasmid mini-prep

T1TE-PMD18T T1T2-pcc010 lacI-pcc009

colony PCR

test pcc009-lacI, no one is right!

Image:PKU Switch 07-8-23 lacI-pcc009.jpg

colony PCR

test RS1, RS2, RD2, SD1, CD1 with GFP-plx007 Image:PKU Switch 07-8-23 double promoters.jpg

Image:PKU Switch 07-8-23 double promoters-2.jpg

some lanes seem to be right, just cultivate them, miniprep and double digestion(XhoI, SalI) tomorrow!

transformation of lacI-pcc009

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