IGEM:Peking/2007/Switch-Notebook/2007-9-4
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Rescue I7100 by gel
Ligation of I7100 with 434OR21/321/EGFP
digestion test of T1TE-T1T2-pcc010
This time I use XhoI and KpnI in M buffer
COlony PCR of I7100-434OR, EGFP
buffer 1.5uL
dNTP 1.5uL
primer 0.5uL each
easy Taq 0.1uL
dH2O 11uL
a total 15uL system
Sequencing
Big day! sequencing result back and we got all fragments right except CD3!
