IGEM:Peking University/2008/Experiment/PKU 0621 G2 1

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TITLE: PCR-pPT1-Gal4-Phusion 50ul*4
Volume (μL)
Template0.5
dNTP2
Enzyme0.5
Buffer10
F-Primer0.2
R-Primer0.2
ddH2O36.6
conditions
TemperatureTime Length(0:00:00)
98°C01:30
98°C00:1030 cycles
60.6,63.1,64.5,68.2°C00:3030 cycles
72°C01:3030 cycles
72°C07:00
4°CHold

TITLE: PCR-Budding Yeast Genome-Gal4-Phusion 50ul*4
Volume (μL)
Template0.5
dNTP2
Enzyme0.5
Buffer10
F-Primer0.2
R-Primer0.2
ddH2O36.6
conditions
TemperatureTime Length(0:00:00)
98°C01:30
98°C00:1030 cycles
60.6,63.1,64.5,68.2°C00:3030 cycles
72°C01:3030 cycles
72°C07:00
4°CHold

TITLE: PCR-pPT-Gal4-Transtart 20ul*6
Volume (μL)
Template0.5
dNTP2
Enzyme0.5
Buffer2
F-Primer0.2
R-Primer0.2
ddH2O14
conditions
TemperatureTime Length(0:00:00)
94°C02:00
94°C01:004 cycles
50°C02:004 cycles
72°C05:004 cycles
94°C01:0030 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C01:0030 cycles
72°C03:0030 cycles
72°C10:00
4°CHold

Ligation-pEasy-T1/Gal4-E.Coli-Amp+,K+(BlueWhiteSelection)
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