IGEM:Peking University/2008/Experiment/PKU 080708 G1 1

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PKU_080708_G1_1



Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pADH-lac1-T

Enzyme cutting-pADH-lac1-T
Volume (μL)
pADH-lac1-T15
SacI5*2
XhoI5
M Buffer10
ddH2O65


Volume (μL)
pADH-lac1-T15
SacI2*2
XhoI2
HindIII2
M Buffer10
ddH2O69



Enzyme cutting-pADH-lac?-AIDwrong
Volume (μL)
pADH-lac?-AIDwrong10
BamHI2*3
XhoI2
K Buffer10
ddH2O76



Enzyme cutting-pGREG504
Volume (μL)
pGREG50410
SacI5
XhoI5
M Buffer10
ddH2O70



Gel running


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