IGEM:Stanford/2009/Project Homeostasis/sequences
From OpenWetWare
Jump to navigationJump to search
<html> <style> /*----- General */ body {
background: black; color: black;
} </style> </html>
<html> <head> <style type="text/css";
color:white;
font-style:italic;
font-family:Arial;
font-size: 1px;
>
</style> </head>
</html>
<html> <head> <style type="text/css";
color:white;
font-style:italic;
font-family:Arial;
font-size: 3px;
>
</style> </head>
</html>
| Project |
| Research Proposal |
| Systems Overview |
| Cloning Plan |
| Sequences & Primers |
| Anti-Inflammation |
| Device Overview |
| Parts Design |
| Challenges |
| Results |
| Anti-Immunosuppression |
| Device Overview |
| Parts Design |
| Challenges |
| Results |
| Protocols |
| Modeling |
| Overview |
| Notebook |
| Results |
| Future Work |
| Archived Work |
Device 1
RBS – SoxR gene – Terminator – Spacer – SoxS Promoter click here
SoxR gene click here
SoxS Promoter click here
RBS - Blh gene click here
RBS - RALDH II gene - Terminator click here
hIL-6 hlyAs click here
Primer Design Guidelines
Resources
- National Center for Biotechnology Information (NCBI)- Get Your Sequences
- Parts Registry of BioBricks- Help with Prefix and Suffix
- IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)
- NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)
- ApE- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements
- Ginkgo BioWorks- creates primers w/ BioBrick additions
Archived Documents and Work
- Past Primers and Sequences
