IGEM:The Citadel/Notebook/Notes Brian/06-23-10
From OpenWetWare
Jump to navigationJump to search
- Re-suspended parts:
- BBa_J23102
- BBa_R0062
- BBa_F2620
- BBa_R0040
- BBa_R0063
- BBa_B0015
- BBa_C0160
- BBa_J45200
- BBa_E0240
- BBa_F1610
- BBa_I20260
- BBa_I13521
- BBa_J45120
- Transformed all parts listed above (electroporation) into TOP10 E.coli.
- Plated 11 part-exposed colonies (100 mL undiluted) onto LB-Amp plates. (I will finish with the remaining 3 tomorrow at 8am and then perform secondary/diluted plates as necessary.)
- Worked with Patrick (haha) on making an open document of all Biobrick DNA currently in use by Citadel iGEM.
- Made LB-Amp plates (38 count).
- Made 2 500mL flasks of LB Miller agar (left in bath overnight since the remaining Ampicillin is in one of the -20°C freezers in Dr. Donnell's [locked] lab.)