IGEM:University of Chicago/2009/Notebook/PNP Biosensor/2009/07/16

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July 16, 2009

Transformations of Longtine PCR Products into Yeast

Transformed products into yeast, however accidentally used Uracil plates instead of -HIS plates for products 1-3. Transformations were unsuccessful for 4 and 5. Beads may spread cell too thinly. Pipet shearing may have also been a cause.

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