IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/06/21

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6/21/10

PCR

Ingredient Amount/Reaction Mastermix (9 reactions)
10X Buffer 4 ul 36 ul
10mM dNTPs 1 ul 9 ul
DNA 2.5 ul 22.5 ul
F Primer .4 ul added individually to each reaction
R primer .4 ul added individually to each reaction
Polymerase .4 ul 3.6 ul
H2O 31.3 ul 281.7 ul

39.2 ul mastermix, .4 ul F primer, .4 ul R primer to each reaction.

Primers at 100uM.

Program: iGEM 2Kb

Samples:

Sample F Primer R Primer
ArsR ArsR F RFC10 ArsR R RFC10
ArsB ArsB F RFC10 ArsB R RFC10
PAL PAL RFC10 F PAL RFC10 R
(-) control H2O H2O




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