IGEM:University of Illinois Urbana Champaign/2009/Notebook/Bioware 2010 Arsenic Bioremediation/2010/08/18
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Incubate at 37 for 15°C min. Inactivate at 80°C for 20 min.
Incubate at room temperature for 10 min, then inactivate at 80°C for 20 min.
Streaked colonies from 8/12/10 plate since I accidentally let them sit at 37°C over the weekend and they may have lost their red color for selection. Streaked 6 colonies from each plate.
50 μL cells, 2 μL ligation product. 10 min on ice, 45 sec at 42°C, 2 min on ice. then add 1 mL SOC, recover for 1 hour at 37°C shaking.
Plated 250 μL cells on Tet plate.