IGEM:VGEM/2007/Notebook/2007-8-3

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  • Made stock solution of 2nd generation K12 cells
  • Made LB control plates
  • Made stock solutions of minimal media and LB
  • Grow up K12 cells from 2nd dilution
  • Began butanol tolerance experiment:
    • Made LB broth solutions containing 100uL of cells and the following butanol concentrations:
    • 0, 0.4, 0.8, 1.2, 1.6, 2.0, 2.4, 2.8, 3.2%
    • Cells went into incubator/shaker at 2PM
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