IGEM:Virginia/2012/Notebook/Genetically engineered bacteriophage for diagnosis of whooping cough/2012/07/01

Week 6	Main project page Previous entry      Next entry
(7/1 – 7/7) Attempted to find antibiotics to which RB53 was resistant to. Performed plasmid miniprep on tube # 3,5,7,10 and then did nanodrop. The nanodrop values were very low, so we added a large volume of template DNA to the PCR reaction. Did PCR clean up on hCG digest. Performed nanodrop on cleaned up, CAIP-treated, and digested PET vector. The nanodrop, however, did not show any DNA present.