JCA082006-Construction of lock3k (J23073)

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Construction of lock3k with 5' extension and resolution of hairpin

PCR ca1028F/ca1001 on pJ23019-J23072 . . .(865 bp, XbaI/PstI)
Sub into pJ23076 . . . . . . . . . . . . .(SpeI/PstI, large 2105)
Product is pJ23019-J23073, [Ptet][lock3k][RFP]

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