JCA111006-iGEM2006Construction100

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J23090

Construction of lock3m with 5' extension and proper RBS spacing

Basically, this is J23071 with the 5' extension

 PCR ca1028F/ca1001 on pJ23019-J23071       (865 bp, XbaI/PstI)
 Sub into pJ23076                           (SpeI/PstI, large 2105)
 Product is pSB3C6-J23090, [Ptet][lock3m][RFP]
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