JL071106-Construction of pSB1A2-J23012

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[SpecR]
PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2)
Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
Product is pSB1A2-J23012


JL1 Forward EcoRI oligo to bioBrick SpecR:
gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc
JL2 Reverse SpeI oligo to bioBrick SpecR:
ccgctactagtCAGCAACGATGTTACGCAGC

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