JonesMA Lab:2xSDS
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2x SDS-PAGE Buffer (10 ml)
1 ml 1M Tris-HCl (pH 6.8)
2 ml 1M DTT
4 ml 10% SDS
1 ml Bromophenol Blue (0.2g in 1 ml ethanol, 9 ml H2O)
2 ml Glycerol
1 ml 1M Tris-HCl (pH 6.8)
2 ml 1M DTT
4 ml 10% SDS
1 ml Bromophenol Blue (0.2g in 1 ml ethanol, 9 ml H2O)
2 ml Glycerol