July

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Tahoura Samad 13:22, 2 July 2010 (EDT)

  • most of my transformations with the new competent cells were successful.
Competent cell Number PDD Growth in initial competent cells Growth in Small Scale Competent Cells Result of Assay with Jin Conclusion
pBca156-Bjh2343123310tons of coloniesNOT DONEtransform new, assay
pBca156-Bjh2343123330tons of coloniesNOT DONEtransform new, assay
pBca156-Bjh2343123481 colony (twice)tons of coloniesNOT DONEtransform new, assay
pBca156-Bjh234312349050-100 coloniesNOT DONEtransform new, assay
pBca156-Bjh2343122910100-200 coloniesNOT DONEtransform new, assay
pBca156-Bjh2343 with BAC72331015 coloniesNOT DONEToday
pBca156-Bjh2343 with BAC7233302 coloniesNOT DONEToday
pBca156-Bjh2343 with BAC7234804 coloniesNOT DONEToday
pBca156-Bjh2343 with BAC7234900DEAD\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2343 with BAC7229100DEAD\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344223310tons of coloniesNOT DONEmake new, transform, assay
pBca156-Bjh2344223330tons of coloniesNOT DONEmake new, transform, assay
pBca156-Bjh23442234812 coloniesTO DOV (UNSTABLE)make new, transform new and old, assay
pBca156-Bjh2344223496 coloniesTO DOV Vmake new, transform new and old, assay
pBca156-Bjh2344222917 coloniesTO DOV Vmake new, transform new and old, assay
pBca156-Bjh2344 with BAC62331tons of coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 with BAC62333tons of coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 with BAC623481 colony (twice)100-200 coloniesNOT DONEToday
pBca156-Bjh2344 with BAC623491 colony(twice)35-50 coloniesX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh2344 with BAC6229135-50 colonies100-200 colonies V V Vtransform new, assay
pBca156-Bjh19343233135-50 coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh193432333<100 coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh193432348tons of coloniesXV Vtransform old, assay
pBca156-Bjh193432349tons of colonies XV Vtransform old, assay
pBca156-Bjh193432291tons of coloniesXV transform old,assay
pBca156-Bjh1934 with BAC52331tons of coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC52333tons of coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC52348tons of colonesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC523491o coloniesXX\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\
pBca156-Bjh1934 with BAC52291tons of coloniesX V V Vtransform old, assay
  • competent cell checks for new 1 and new 6 looked good.
  • miniprepped IBB as CA lefty, CK lefty and CK righty, and sent out for sequencing with ca998.

Tahoura Samad 13:38, 1 July 2010 (EDT)

  • controls for JTK030 looked good with no growth on Kan, Amp, Cam, good growth on LB, and a little growth on Spec, which is the normal result because of mutations in the bacteria. Those are labeled with double black stripes, in a box in the back of the -80 in the 2nd row from the top.

Kan
LB
Cam
Amp
Spec

  • picked and grew up the results of PCA for the new nuclear localization tag. They had plenty of colonies, with only one background green colony. Will miniprep them tomorrow.
  • did small scale competent cell prep of 1 and 6.
  • did the following transformations (1old:2348), (5 old:2291) (6 old:2291) (6 new:2291),(3 old :2348) (3 old: 2349) (3 old:2291) (2 old:2348) (2 old: 2349)(2 old:2291) (2 new:2348) (2 new:2349) (2 new: 2291), (1 new:2331) (1 new:2333) (1 new:2348) (1 new:2349) (1 new: 2291) (6 new 2348) (6 new:2349), (IBB into lefty).
  • redid assembly of stage 0 of life act but with 2.4 μL of each DNA and 1.2 μL of 5x NEB2.
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