KAD062806-Construction of J23006-J23007
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Klenow Extend KB003 and KB004 (65bp, XbaI/PstI, Neb2)
Paste into J023006 (XbaI/PstI, Neb2, Large 2334bp)
Product is J23006-J23007
Forward XbaI oligo for part 3b:
ctccatctagagACCCAAAAGCAAGAGGTGATTCTAGTTtac
reverse PstI oligo for part 3b:
gcgaactgcagcggccgctactagtaAACTAGAATCACCTCTTG
