Kafatos:Making 1kb+ Ladder Stock

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Overview

Making 1kb+ DNA Ladder Stock. We dilute the entire contents of the tube and make 5x 500uL aliquots

Materials

  1. 250uL 1kb+ DNA Ladder [1ug/uL] (Invitrogen)
  2. 500uL 5x DNA Loading Buffer
  3. 1750uL AccuGene Water (Lonza)

Procedure

  1. Add the 3 components above together
  2. Mix well and make 5 x 500uL aliquots
  3. Leave one working stock at 4C and keep the other frozen at -20C
  4. Use 10uL of this stock per lane (1ug of ladder per lane)

Notes

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