Klapperich Lab:Pressing Zeonor Slides for DNA Growth

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Contents

Overview

Press Zeonor pellet into 1mm slide.

Materials

  • Carver Hot Press
  • Mass Scale
  • Zeonor 750 pellets
  • 0.8mm shims

Procedure

  • Mass out 5900 mg Zeonor pellets
  • Spread in uniform circle on Hot Press slide
  • Apply three orange 0.8mm shims in this configuration:
Fig1. Diagram of Shims Layout
  • Press at 2000 psi for 5-6minutes

Notes

  1. List troubleshooting tips here.
  2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
  3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding ('''~~~~''') to the end of your tip.

References

Relevant papers and books

  1. Goldbeter A and Koshland DE Jr. . pmid:6947258. PubMed HubMed [Goldbeter-PNAS-1981]
  2. JACOB F and MONOD J. . pmid:13718526. PubMed HubMed [Jacob-JMB-1961]
  3. Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164. [Ptashne-Genetic-Switch]
All Medline abstracts: PubMed HubMed

Contact

  • Who has experience with this protocol?
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