Klapperich Lab:Pressing Zeonor Slides for DNA Growth
Press Zeonor pellet into 1mm slide.
- Carver Hot Press
- Mass Scale
- Zeonor 750 pellets
- 0.8mm shims
- Mass out 5900 mg Zeonor pellets
- Spread in uniform circle on Hot Press slide
- Apply three orange 0.8mm shims in this configuration:
- Press at 2000 psi for 5-6minutes
- List troubleshooting tips here.
- You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
- Anecdotal observations that might be of use to others can also be posted here.
Please sign your name to your note by adding ('''~~~~''') to the end of your tip.
Relevant papers and books
- Goldbeter A and Koshland DE Jr. . pmid:6947258.
- JACOB F and MONOD J. . pmid:13718526.
- Mark Ptashne. A genetic switch. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 2004. isbn:0879697164.
- Who has experience with this protocol?