Knight:Primers

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Knight Lab



List any primers that might be generally useful here. These should be locatable in the "common primers" box.

  • VF2: 5' tgc cac ctg acg tct aag aa 3'
  • VR: 5' ATT ACC GCC TTT GAG TGA GC 3'
  • VF2 (reverse): 5' TTC TTA GAC GTC AGG TGG CA 3'
  • VR (forward): 5' GCT CAC TCA AAG GCG GTA AT 3'
  • BioBricks-f: 5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
    • ordered by Reshma 5/1/2006 (25μM)
  • BioBricks-r: 5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
  • Suffix-F: 5' A CTA GTA GCG GCC GCT GCA G 3'
  • Prefix-R: 5' T CTA GAT GCG GCC GCG AAT TC 3'
  • Suffix-F long: 5' GTT TCT TCA CTA GTA GCG GCC GCT GCA G 3'
  • Prefix-R long: 5' GTT TCT TCT CTA GAT GCG GCC GCG AAT TC 3'
  • M13-R: 5' cag gaa aca gct atg ac 3'
  • M13-F (-20): 5' GTA AAA CGA CGG CCA G 3'
  • T7 terminator: GCTAGTTATTGCTCAGCGG (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • lacI-R: gtataacgttactggtttcac (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • pET upstream: atgcgtccggcgtaga (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • DuetUp2: ttgtacacggccgcataatc (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • DuetDOWN1 GATTATGCGGCCGTGTACAA (50μM) --Austin Che 13:55, 30 August 2007 (EDT)
  • T7-VF2: GTTTCTGCtaatacgactcactatagggagaTGCCACCTGACGTCTAAGAA (50μM) --Austin Che 10:39, 3 September 2007 (EDT)
  • T7-VR: GTTTCTGCtaatacgactcactatagggagaATTACCGCCTTTGAGTGAGC (50μM) --Austin Che 10:39, 3 September 2007 (EDT)
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