Knight:Protein DNA binding/Option 1/quick reference recipe

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To make 1mL ...

  • 100μL 50% glycerol
  • 50μL 1M potassium glutamate
  • 30μL 500mM HEPES-NaOH (pH7.9)
  • 10μL 10% NP-40
  • 10μL 5M potassium acetate
  • 12.5μL 4M potassium chloride
  • 5μL 1M magnesium chloride
  • 1μL 20mM zinc sulfate
  • 2μL 1mg/mL BSA
  • 779.5μL H2O

To make 1000mL ...

  • 100mL 50% glycerol
  • 50mL 1M potassium glutamate
  • 30mL 500mM HEPES-NaOH (pH7.9)
  • 10mL 10% NP-40
  • 10mL 5M potassium acetate
  • 12.5mL 4M potassium chloride
  • 5mL 1M magnesium chloride
  • 1mL 20mM zinc sulfate
  • 200μL 10mg/mL BSA
  • 781.3mL H2O
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