Koch Lab:Protocols/ATP

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May 2009

Steve Koch 14:46, 12 May 2009 (EDT): Anthony was able to get 1 M Tris-HCl, pH 7.5 and 1M MgCl2 from Kelly (thanks, Kelly!).

  • Had previously added 100 ul of water to ATP. Was supposed to add 1 ml of 100 mM Tris pH 7.5, so, need to add:
    • 800 ul of cold water
    • 100 ul of 1 M Tris.

Final concentration 100 mM ATP in 100 mM Tris-HCl Ph 7.5 Stock lyophilized powder is from cytoskeleton

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