Lauffenburger:Brian Joughin

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Brian A. Joughin

Research Scientist, The David H. Koch Institute for Integrative Cancer Research at MIT

Email

Current Research

  • Examination of the interpositional dependence of kinase specificity on substrate sequence.
  • Development of a bioinformatic method for discovering substrate motif specificity of uncharacterized kinases by integration of existing data.
  • Deriving biological insight from high-throughput quantitative phosphoproteomic mass spectrometry data.

Education

  • 2007, Ph.D., Department of Biology, MIT
    • Research Advisors: Profs. Bruce Tidor and Michael B. Yaffe
    • Doctoral thesis: "Novel Methods in Computational Analysis and Design of Protein-Protein Interactions: Applications to Phosphoregulated Interactions"
  • 2000, B.A., Department of Biophysics, Johns Hopkins University
    • Research Advisor: Prof. Eaton Lattman

Publications

  1. Joughin BA, Liu C, Lauffenburger DA, Hogue CWV, Yaffe MB. Protein kinases display minimal interpositional dependence on substrate sequence: potential implications for the evolution of signalling networks. Phil. Trans. R. Soc. B. 2012 Sep 19. 367 2574-2583;
  2. Grosstessner-Hain K, Hegemann B, Novatchkova M, Rameseder J, Joughin BA, Hudecz O, Roitinger E, Pichler P, Kraut N, Yaffe MB, Peters JM, Mechtler K. Quantitative phospho-proteomics to investigate the Polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics. 2011 Nov;10(11):M111.008540. Epub 2011 Aug 21. Abstract
  3. Alexander J, Lim D, Joughin BA, Hegemann B, Hutchins JRA, Ehrenberger T, Ivins F, Sessa F, Hudecz O, Nigg EA, Fry AM, Musacchio A, Stukenberg PT, Mechtler K, Peters J-M, Smerdon S-J, Yaffe MB, Spatial Exclusivity Combined with Positive and Negative Selection of Phosphorylation Motifs Is the Basis for Context-Dependent Mitotic Signaling. Sci. Signal. 4, ra42 (2011). Abstract
  4. Naegle KM, Gymrek M, Joughin BA, Wagner JP, Welsch RE, Yaffe MB, Lauffenburger DA, White FM. PTMScout: A web resource for analysis of high-throughput post-translational proteomic studies. Mol Cell Proteomics. 2010 Nov;9(11):2558-70. Epub 2010 Jul 14. Abstract PTMScout
  5. Joughin BA, Cheung E, Karuturi RK, Saez-Rodriguez J, Lauffenburger DA, Liu ET. Cellular Regulatory Networks in Systems Biomedicine: Concepts and Perspectives. Liu ET and Lauffenburger DA, eds., Academic Press. pp. 57-108 (2009). Link
  6. Joughin BA, Naegle KM, Huang PH, Yaffe MB, Lauffenburger DA, White FM. An integrated comparative phosphoproteomic and bioinformatic approach reveals a novel class of MPM-2 motifs upregulated in EGFRvIII-expressing glioblastoma cells. Mol Biosyst. 2009 Jan;5(1):59-67. Epub 2008 Oct 30. Abstract
  7. Joughin BA, Green DF, Tidor B. Action-at-a-distance interactions enhance protein binding affinity. Protein Sci. 2005 May;14(5):1363-9. Epub 2005 Mar 31. Abstract
  8. Joughin BA, Tidor B, Yaffe MB. A computational method for the analysis and prediction of protein:phosphopeptide-binding sites. Protein Sci. 2005 Jan;14(1):131-9. Epub 2004 Dec 2. Abstract
  9. Powell DW, Rane MJ, Joughin BA, Kalmukova R, Hong JH, Tidor B, Dean WL, Pierce WM, Klein JB, Yaffe MB, McLeish KR. Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding. Mol Cell Biol. 2003 Aug;23(15):5376-87. Abstract
  10. Pereira de Araujo AF, Pochapsky TC, Joughin B. Thermodynamics of interactions between amino acid side chains: experimental differentiation of aromatic-aromatic, aromatic-aliphatic, and aliphatic-aliphatic side-chain interactions in water. Biophys J. 1999 May;76(5):2319-28. Abstract
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