Lidstrom:Pouring Media Plates

From OpenWetWare

Jump to: navigation, search

Back to Protocols

Critical:

  • Put date on plates. Some antibiotics aren't very long lasting.
    • For example, Ampicillin plates only last 2-3 weeks. (is there a citation for this? -Janet 2012/07/03)
  • Wait to put the antibiotic in until it is cool enough to touch the bottle to your cheek.
    • This prevents thermal degradation.
  • If you tend to get bubbles appearing in the agar plates after incubating at 37oC:
    • Let the media cool more before pouring plates
    • "plates that have been stored in the refrigerator should be separated on a bench top and allowed to warm gradually to room temperature for several hours before incubating at 37 °C." -- Sturdier 2014

Drew Endy has all the basics here

Personal tools