- Get liquid media (over bench). Choose the right liquid media for your yeast.
- Put 5 mL of liquid media in culture tubes.
- Be sterile!
- Get yeast from plate in 4 degree fridge.
- Swipe off 1 colony (far from other colonies) with a wooden stick.
- Swish stick in culture with yeast on walls of tube.
- Vortex briefly.
- Put in 30 degree room overnight on the roller with speed 7ish.