MES

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2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 [1][2][3]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.

• pK_a at 25C of 6.10 (5.97 at 37C)
• usable buffering range of 5.5 to 6.7
• molecular weight 195.24
• ΔpK_a/ΔT = -.011

Buffers are typically 1 M, prepared by neutralizing MES with sodium hydroxide.

1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry 1966 Feb; 5(2) 467-77. pmid:5942950. PubMed HubMed [Good66]
2. Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol 1972; 24 53-68. pmid:4206745. PubMed HubMed [Good74]
3. Blanchard JS. Buffers for enzymes. Methods Enzymol 1984; 104 404-14. pmid:6717292. PubMed HubMed [Blanchard84]