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2--(N-morpholino)ethanesulfonic acid, CAS 4432-31-9 [1, 2, 3]. Stable pH vs. temperature, no primary amine groups, no metal chelation, near physiologic pH range.

  • pKa at 25°C of 6.10 (5.97 at 37°C)
  • usable buffering range of 5.5 to 6.7
  • molecular weight 195.24
  • ΔpKa/ΔT = -.011

Buffers are typically 1 M, prepared by neutralizing MES with sodium hydroxide.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. . pmid:5942950. PubMed HubMed [Good66]
  2. Good NE and Izawa S. . pmid:4206745. PubMed HubMed [Good74]
  3. Blanchard JS. . pmid:6717292. PubMed HubMed [Blanchard84]
All Medline abstracts: PubMed HubMed and
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