Magni:Protocols/ph

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pH adjustment for LB medium

  • Before autoclaving the LB medium:
    • dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
    • ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
    • wash the pH probe with deionized water and store it in KCl 3M.
  • Autoclave the medium

pH adjustment for M9 medium

  • According to the M9 medium preparation protocol, mix:
    • water,
    • glycerol and
    • M9 salts.
  • Dip the pH-meter probe in the mix to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
  • ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
  • wash the pH probe with deionized water and store it in KCl 3M.
  • Autoclave the mix in a bottle.
  • Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4).
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