Maheshri:SOPs-prot
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Chromosome Conformation Capture (3C)
Author: Richard Joh
Effective date: Mar 27 2012
Description: The chemical fume hood is designed to provide a safe environment for working with noxious and/or volatile chemicals. It is not designed to provide an environment for conducting biological experiments.
Potential Safety Issues: Chemical exposure from improper operation.
Startup Procedure:
- Ensure that air is flowing through the hood prior to use.
- Turn the light on in the hood using the light switch found on the left side of the hood.
- Lift the hood’s sash up 9-12 inches to allow room to work in the hood.
Shutdown Procedure:
- Ensure that all chemicals are put away and the hood is clean.
- Lower the hood’s sash to about 1-2 inches above the floor of the hood.
- Turn on the hood’s light using the light switch found on the left side of the hood.
In the Event of a Power or Airflow Loss: Discontinue use of the hood and shut it down.
Monitoring Required: The chemical hood must be inspected and validated annually. The chemical hood is also inspected weekly by the student safety coordinator.