Maloof Lab:PCR stuff

From OpenWetWare

Jump to: navigation, search

Image:logo2_final.jpg

Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

Contact

Home      Research      Publications      Protocols      Resources      Announcements      Lab Safety     

Important: Label tubes with buffer name, date, your initials, and tube number

Contents

10x PCR buffer (see Promega PCR buffer) for 20 ml

500 mM KCl (10 ml 1M stock)
100 mM Tris/HCl (pH 9.0) (2 ml 1M stock)
15 mM MgCl2 (0.3 ml 1M stock)
1% Triton X (1 ml 10% Triton X)

Add sterile MilliQ up to 20 ml in total.

Note 1: Renew 10xPCR buffer every 1~2 months.


Taq polymerase

1 (current ('07) stock Taq) : 9 (storage buffer)

MgCl2 (25 mM)

Add 250 µl 1M MgCl2 to 10 ml dH2O

300 µl aliquots in 0.5 ml tubes.


dNTP (100mM total, 25 mM each)

Add equal amount of dATP, dTTP, dCTP, dGTP (100 mM, Fermentas dNTP Set #R0186)

Make 100 µl aliquots.

Personal tools