Maloof Lab:Seed Sterilization

From OpenWetWare

Jump to: navigation, search

Image:logo2_final.jpg

Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

Contact

Home      Research      Publications      Protocols      Resources      Announcements      Lab Safety     

Ethanol Seed Sterilization


  1. Tap seeds into 1.5mL tubes (labeled with VWR pen)
  2. Add 1mL 70% Ethanol, 0.1% Triton solution to each tube
  3. Vortex at 8 for 5 minutes
  4. Aspirate liquid off of seeds
  5. Add about 1mL 70% Ethanol to each tube
  6. In sterile hood, pipette seeds onto filter paper (labeled with pencil)
  7. Let Ethanol evaporate away from filter paper
  8. When filter paper is dry, tap seeds onto plates
  9. Tape plates, wrap in foil, and refrigerate at 4 degrees for 3-4 days before unwrapping and placing plates in growth chamber
Personal tools