This protocol will make 1 liter of 50x solution. Adjust to your needs if necessary.
- Add 242 g of Tris base
- 57.1 ml of glacial acetic acid
- 100 ml of 0.5M EDTA (pH 8.0)
- Add water to 1 liter
This protocol will make 2 liter of 50x solution. Adjust to your needs if necessary.
- Add 40 g of NaOH into about 1.8 liters of H2O
- pH to 8.0 with Boric Acid (about 180 g)
- Add water to 2 liters