Maloof Lab:buffers electrophoresis

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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50x TAE


This protocol will make 1 liter of 50x solution. Adjust to your needs if necessary.

  1. Add 242 g of Tris base
  2. 57.1 ml of glacial acetic acid
  3. 100 ml of 0.5M EDTA (pH 8.0)
  4. Add water to 1 liter

50x SB


This protocol will make 2 liter of 50x solution. Adjust to your needs if necessary.

  1. Add 40 g of NaOH into about 1.8 liters of H2O
  2. pH to 8.0 with Boric Acid (about 180 g)
  3. Add water to 2 liters


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