Maloof Lab:gelred loading dye

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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Contents

6x Xylene Cyanol Loading Buffer for Electrophoresis


This buffer will have only the light blue than runs slower on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.

  1. 100mg Xylene-cyanol
  2. 6g of Ficoll (Type 400)
  3. Add water to 40 ml

Dissolve well. It takes a while. An option is to shake at 37 degrees overnight.

6x Bromophenol Blue Loading Buffer for Electrophoresis


This protocol will make 2 liter of 50x solution. Adjust to your needs if necessary.

This buffer will have only the dark blue than runs faster on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.

  1. 100mg Bromophenol Blue
  2. 6g of Ficoll (Type 400)
  3. Add water to 40 ml

Dissolve well. It takes a while. An option is to shake at 37 degrees overnight.


Loading Buffer with GelRed for Electrophoresis


To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.

  1. 5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)
  2. 15 ul of GelRed (10000x in water, cat.41003 Biotium)


1 Kb Ladder with GelRed for Electrophoresis


Use the Fermentas GeneRuler 1kb ladder from the stock. Mix:

  1. 100µl (1 tube) DNA Ladder
  2. 100µl 6X Loading Dye Solution with GelRed (from above)
  3. 400µl Deionized water

Good to go. Load 3 to 5 µl.

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