Matt Gethers/CRI, Thailand/Labwork/Digests/NdeI and BamHI Double Digest/8.5.08 Mixes
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| Reagent
|
Volume
|
| pET-11a |
1 μl
|
| NdeI |
0 μl
|
| BamHI |
0 μl
|
| Fast Digest Buffer |
2 μl
|
| H20 |
17.0 μl
|
| Total |
20 μl
|
| Reagent
|
Volume
|
| pET-11a |
1 μl
|
| NdeI |
0.5 μl
|
| BamHI |
0 μl
|
| Fast Digest Buffer |
2 μl
|
| H20 |
16.5 μl
|
| Total |
20 μl
|
| Reagent
|
Volume
|
| pET-11a |
1 μl
|
| NdeI |
0 μl
|
| BamHI |
0.5 μl
|
| Fast Digest Buffer |
2 μl
|
| H20 |
16.5 μl
|
| Total |
20 μl
|
| Reagent
|
Volume
|
| pET-11a |
10 μl
|
| NdeI |
0.5 μl
|
| BamHI |
0.5 μl
|
| Fast Digest Buffer |
2 μl
|
| H20 |
7.0 μl
|
| Total |
20 μl
|
| Reagent
|
Volume
|
| 8.2.08 HmgR ORF |
10 μl
|
| NdeI |
0.5 μl
|
| BamHI |
0.5 μl
|
| Fast Digest Buffer |
2 μl
|
| H20 |
7.0 μl
|
| Total |
20 μl
|
