Matt Gethers/CRI, Thailand/Labwork/Polyacrylamide Gels/Week of 7.7.08
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Week of 7.7.08
7.8.08
Poured gel and prepared samples according to protocol. My samples were 1 ml of the 200 ml culture used for isolation of HmgR before induction and 1 ml of the same culture after induction. Because I had only two samples, I loaded, 5, 10, and 20 μl of each.
Gel looks good - protein appears in induced lanes at the correct location (~28 kDa) and not in uninduced lanes. Presence also increases with concentration.
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | |
| 5 μl Unstained protein marker | 5 μl uninduced cell sample | 5 μl induced cell sample (didn't get into well) | 5 μl induced cell sample (did get into well) | 10 μl uninduced cell sample | 10 μl induced cell sample | 20 μl uninduced cell sample | 20 μl uninduced cell sample (a little from adjacent lane leaked into well) | 20 μl induced cell sample (did get into well) |
