Matt Gethers/CRI, Thailand/Specifications/pIs001/36.SEQ
From OpenWetWare
Jump to navigationJump to search
36.SEQ (pIs001.P1, T7_rev repeat)
36.SEQ (pIs001.P1, T7_rev repeat)
Redundant Sequencing: 33.SEQ
Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number + 189 where 189 is the number of trimmed 5' bases.
Base Number (Vector NTI File Annotation) = 5986 - Contig Sequence File Annotation Number
| Base Number (Sequence File Annotation) | Base Number (Vector NTI File Annotation) | Theoretical Base | Read Base | Notes |
| 202 | 5974 | C | N | Could be A or C - Not sure |
| 204 | 5972 | G | N | Could be G or A - Not Sure |
| 223 | 5953 | C | T | Looks like a C - good |
| 304 | 5872 | C | N | Maybe a C - Not Sure |
| 309 | 5867 | G | N | Could be G or T - Not Sure |
| 321 | 5855 | C | N | Maybe an A or C - Not Sure |
| 333 | 5843 | G | N | Maybe a G or an A - Not Sure, but G confirmed by other read |
| 342 | 5834 | G | A | Looks like an A, but could be a G, G confirmed by other read other read |
| 346 | 5830 | G | Space | Looks like there could be a G there, G confirmed by other read other read |
Notes
Region of Interest (Vector NTI Annotation): 5318 - 6121 (ATG to TGA)
Lots of truncated 5' and 3' bases - may want to redo alignment with less trimming/manual comparison. Considering the 189 trimmed 3' bases would allow me to complete the region between the forward and reverse primers (~50 bases between the two) while considering the 188 trimmed 5' bases would allow me to see the end of the ORF (~140 bases to end of ORF).
