Matt Gethers/CRI, Thailand/Specifications/pIs001/37.SEQ

37.SEQ (pIs001.P2, T7_rev repeat)

37.SEQ (pIs001.P2, T7_rev repeat)

PIs001P2contig.cep

Base Number (Sequence File Annotation) = Contig Sequence File Annotation Number + 65 where 64 is the number of trimmed 5' bases (not sure why I have to add 1).

Base Number (Vector NTI File Annotation) = 6108 - Contig Sequence File Annotation Number

Base Number (Sequence File Annotation)	Base Number (Vector NTI File Annotation)	Theoretical Base	Read Base	Notes
77	6096	G	A	Could be a G - good
83	6090	G	N	Could be an A or G - Not sure
86	6087	G	N	Could be an A or G - Not sure
130	6043	Space	T	Definitely an A there - problem
136	6037	C	N	Looks like a C - good
139	6034	G	N	Looks like a G - good
140	6033	G	T	Probably a G - good
141	6032	A	N	Looks like a A - good
164	6009	C	N	Looks like a C - good
176	5997	C	N	C or A - Not sure
177	5996	T	N	C or T - Not sure
179	5994	Space	T	Definitely signal for a T there - problem
185	5988	G	T	Could be a G or a T - Not Sure
187	5986	C	N	Could be C or G - Not sure
188	5985	G	A	Could be a G, but strong signal for A - Not Sure
189	5984	G	T	Could be a G, but could be a T - Not Sure
201	5972	C	N	Probably a C - good
222	5951	C	T	Likely a C - good
308	5865	G	N	Likely a G - good
339	5834	C	T	Definitely A, no sign of C - problem
379	5794	C	N	Likely a C - good
393	5780	C	N	C or A - Not Sure
398	5775	G	Space	Looks like a G was missed, G also confirmed by other read - good
				From 414 (5759) on, there are 5 mismatched spaces, but the base is confirmed by the forward primer - good.

Notes

This read isn't as clean as the forward primer: some points of concern.