McClean: Yeast Dubious ORFs

Overview

List of dubious orfs where S. Silverman (Botstein Lab, Princeton) would insert things into the yeast genome. Picked (according to Sandy) based on lack of “things” 3’ and 5’ that could be messed up and because they lack any known function/effect on viability.

List of ORFs

• YAR030C
• YBR032W
• YBR144C
• YDL242W
• YDR426C
• YDR445C
• YIL028W
• YKL055W
• YMR052C-A
• YOL118C
• YOR0115W

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

1. List troubleshooting tips here.
2. You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
3. Anecdotal observations that might be of use to others can also be posted here.

Please sign your name to your note by adding '''*~~~~''': to the beginning of your tip.

References

S. Silverman, personal communication

Contact

• Megan N McClean 14:01, 05 July 2015 (EDT)

or instead, discuss this protocol.