Mesoplasma florum:Tetracycline resistance

Mutations of 16S region conveying Tetracycline resistance

• G1058C PMID 9661007 (P. acne)
• G92T
• T93-
• C95G
• T96G
• G360A
• G741A
• A782C
• C883G
• A965G PMID 12435699, PMID 15901694 (H. pylori)
• G966T
• A967C
• G1026A
• C1033T
• T1136C
• C1167T
• A1299G
• C1293T

Alignment of E. coli 16S with M. florum 16S sequences MF GENBANK AF300327

    1 EC         AAATTGAAGA GTTTGATCAT GGCTCAGATT GAACGCTGGC GGCAGGCCTA
      MF         ---------- --------CT GGCTCAGGAY GAACGCTGGC GGCATGCCTA

   51 ACACATGCAA GTCGAACGGT AACAGGAAGA AGCTTGCTTC TTTGCTGACG AGTGGCGGAC
      ATACATGCAA GTCGAACGG- -------AGG TGCTTGCACC TC-------- AGTGGCGAAC

  111 GGGTGAGTAA ----TGTCTG GGAAACTGCC TGATGGAGGG GGATAACTAC TGGAAACGGT
      GGGTGAGTAA CACGTATCT- --AATCTACC TTCTAGCGGG GGATAACTTT TGGAAACGAA

  167 AGCTAATACC GCA------- --TAACGTCG CAAGACCAAA ----GAGGGG GACCTTCGGG
      AGGTAATACC GCATGTGGAT GTTATTATCG CATGAGAAAA CATTCAAAGA TCCGTTTGGA

  214 CCTCTTGCCA TC--GGATGT GCCCAGATGG GATTAGCTAG TAGGTGGGGT AACGGCTCAC
      TCACTAGAAG ATGAGGATGC GGC------G TATTAGCTAG TAGGCGGGGT AAAGGCCCAC

  272 CTAGGCGACG ATCCCTAGCT GGTCTGAGAG GATGACCAGC CACACTGGAA CTGAGACACG
      CTAGGCGATG ATACGTAGCC GAACTGAGAG GTTGATCGGC CACATTGGGA CTGAGATACG

  332 GTCCAGACTC CTACGGGAGG CAGCAGTGGG GAATATTGCA CAATGGGCGC AAGCCTGATG
      GCCCAGACTC CTACGGGAGG CAGCAGTAGG GAATTTTTCA CAATGGACGA AAGTCTGATG

  392 CAGCCATGCC GCGTGTATGA AGAAGGCCTT CGGGTTGTAA AGTACTTTCA GCGGGGAGGA
      AAGCAATGCC GCGTGAGTGA TGACGGCCTT CGGGTTGTAA AGCTCTGTTG TAAGGGAAGA

  452 AG--GGAGTA AAGTTAATAC CTTTGCTCAT TGACGTTACC CGCAGAAGAA GCACCGGCTA
      AAACATAGGA GAGGAAATGC TCTTATC--T TGACGGTACC TTACCAGAAA GCCACGGCTA

  510 ACTCCGTGCC AGCAGCCGCG GTAATACGGA GGGTGCAAGC GTTAATCGGA ATTACTGGGC
      ACTATGTGCC AGCAGCCGCG GTAATACATA GGTGGCAAGC GTTATCCGGA TTTATTGGGC

  570 GTAAAGCGCA CGCAGGCGGT TTGTTAAGTC AGATGTGAAA TCCCCGGGCT CAACCTGGGA
      GTATAGGGTG CGTAGGCGGT TTCGCAAGTT TGAGGTTAAA GCCCGGAGCT CAACTCCGGT

  630 ACTGCATCTG ATACTGGCAA GCTTGAGTCT CGTAGAGGGG GGTAGAATTC CAGGTGTAGC
      TC-GCCTTGA AAACTGCGGG ACTAGAATAT CAGAGAGGTA AGCGGAATTC CATGTGTAGC
 
  690 GGTGAAATGC GTAGAGATCT GGAGGAATAC CGGTGGCGAA GGCGGCCCCC TGGACGAAGA
      GGTAAAATGC GTAGATATAT GGAAGAACAC CAGTGGCGAA AGCGGCTTAC TGGCTGATTA

  750 CTGACGCTCA GGTGCGAAAG CGTGGGGAGC AAACAGGATT AGATACCCTG GTAGTCCACG
      TTGACGCTGA GGCACGAAAG CGTGGGGAGC AAATAGGATT AGATACCCTA GTAGTCCACG

  810 CCGTAAACGA TGTCGAC--- ---TTGGAGG TTGTGCCCTT GAGGCGTGGC TTCCGGAGCT
      CCGTAAACGT TGAGTACTAA GTATTGGGGA TTACCTCAGT G--------- --CTGCAGCT

  864 AACGCGTTAA GTCGACCGCC TGGGGAGTAC GGCCGCAAGG TTAAAACTCA AATGAATTGA
      AACGCATTAA GTACTCCGCC TGAGTAGTAT GCTCGCAAGA GTGAAACTCA AAGGAATTGA

  924 CGGGGGCCCG CACAAGCGGT GGAGCATGTG GTTTAATTCG ATGCAACGCG AAGAACCTTA
      CGGGGACCCG CACAAGTGGT GGAGCATGTG GTTTAATTCG AAGCAACACG AAGAACCTTA

  984 CCTGGTCTTG ACATCCA--- CAGAACTTTC CAGAGATGGA TTGGTGCCTT CGGGAACTGT
      CCAGGGCTTG ACATACAGTG CAAAGC--TA CAGAGATGTA GTGGAGGTT- ----AACATT

 1041 GAGACAGGTG CTGCATGGCT GTCGTCAGCT CGTGTTGTGA AATGTTGGGT TAAGTCCCGC
      GATACAGGTG GTGCATGGTT GTCGTCAGTT CGTGCCGTGA GGTGTTGGGT TAAGTCCCGC

 1101 AACGAGCGCA ACCCTTATCT TTTGTTGCCA GCGGTCCGGC CGGGAACTCA AAGGAGACTG
      AACGAACGCA ACCCTTGTCG TTAGTTACTA AC-ATTAAGT TGAGGACTCT AACGAGACTG

 1161 CCAGTGATAA ACTGGAGGAA GGTGGGGATG ACGTCAAGTC ATCATGGCCC TTACGACCAG
      CTAGTG-TAA GCTAGAGGAA GGTGGGGATG ACGTCAAATC ATCATGCCCC TTATGTCCTG

 1221 GGCTACACAC GTGCTACAAT GGCGCATACA AAGAGAAGCG ACCTCGCGAG AGCAAGCGGA
      GGCTACACAC GTGCTACAAT GGCCGATACA AAGAGTCGCA ATCTCGCGAG GGGGAGCTAA

 1281 CCTCATAAAG TGCGTCGTAG TCCGGATTGG AGTCTGCAAC TCGACTCCAT GAAGTCGGAA
      TCTCAAAAAG TCGGTCTCAG TTCGGATTGA AGTCTGCAAC TCGACTTCAT GAAGCCGGAA

 1341 TCGCTAGTAA TCGTGGATCA GA-ATGCCAC GGTGAATACG TTCCCGGGCC TTGTACACAC
      TCACTAGTAA TCGCGAATCA GCTATGTCGC GGTGAATACG TTCTCGGGTC TTGTACACAC

 1400 CGCCCGTCAC ACCATGGGAG TGGGTTGCAA AAGAAGTAGG TAGCTTAACC TTCGGGAGGG
      CGCCCGTCAA ACCACGAGAG TTGGTAATAC CAGAAGTACG TTTCCTAACC GTAAGGAAGG

 1460 CGCTTACCAC TTTGTGATTC ATGACTGGGG TGAAGTCGTA ACAAGGTAAC CGTAGGGGAA
      CGCGTCCCAA GGTAGGACTA GCGATTGGGG TTAAGTCGTA ACAAGGTATC CGTACGGGAA

 1520 CCTGCGGTTG GATCACCTCC TTA        1542
      CGTGCGGATG GATCAC---- ---

Canonical numbering of 16S ribosomal RNA sequence is keyed off of the E. coli sequence, given here in FASTA format and in Genbank format. This sequence is from GENBANK U00096, bases 223771-225312.

>gi|48994873:223771-225312 Escherichia coli K12 MG1655, complete genome
AAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT
AACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGAT
GGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTC
GGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGAC
GATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAG
GCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT
TCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCC
GCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAA
TTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAA
CTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCG
TAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGC
GTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCC
CTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAAC
TCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACC
TTACCTGGTCTTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTG
CTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCT
TTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG
ACGTCAAGTCATCATGGCCCTTACGACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCG
ACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCAT
GAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACC
GCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACT
TTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCT
TA

       1 aaattgaaga gtttgatcat ggctcagatt gaacgctggc ggcaggccta acacatgcaa
      61 gtcgaacggt aacaggaaga agcttgcttc tttgctgacg agtggcggac gggtgagtaa
     121 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
     181 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
     241 ggattagcta gtaggtgggg taacggctca cctaggcgac gatccctagc tggtctgaga
     301 ggatgaccag ccacactgga actgagacac ggtccagact cctacgggag gcagcagtgg
     361 ggaatattgc acaatgggcg caagcctgat gcagccatgc cgcgtgtatg aagaaggcct
     421 tcgggttgta aagtactttc agcggggagg aagggagtaa agttaatacc tttgctcatt
     481 gacgttaccc gcagaagaag caccggctaa ctccgtgcca gcagccgcgg taatacggag
     541 ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac gcaggcggtt tgttaagtca
     601 gatgtgaaat ccccgggctc aacctgggaa ctgcatctga tactggcaag cttgagtctc
     661 gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg tagagatctg gaggaatacc
     721 ggtggcgaag gcggccccct ggacgaagac tgacgctcag gtgcgaaagc gtggggagca
     781 aacaggatta gataccctgg tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc
     841 cttgaggcgt ggcttccgga gctaacgcgt taagtcgacc gcctggggag tacggccgca
     901 aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat
     961 tcgatgcaac gcgaagaacc ttacctggtc ttgacatcca cagaactttc cagagatgga
    1021 ttggtgcctt cgggaactgt gagacaggtg ctgcatggct gtcgtcagct cgtgttgtga
    1081 aatgttgggt taagtcccgc aacgagcgca acccttatct tttgttgcca gcggtccggc
    1141 cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg acgtcaagtc
    1201 atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca aagagaagcg
    1261 acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg agtctgcaac
    1321 tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt
    1381 tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt
    1441 agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt gaagtcgtaa
    1501 caaggtaacc gtaggggaac ctgcggttgg atcacctcct ta