NanoBio:FPLC Methods

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FPLC Methods

CAjoFmethodHis

  • Frac-950: serpentine row
  • Variables:
    • Main:Column: HisTrap_FF_5mL
    • Start w/ Pump Wash:
      • Wash Inlet A: OFF
      • Wash Inlet B: OFF
    • Flow Rate: Flow Rate (mL/min): 0.7
    • Column Pressure Limit (MPa): 0.3
    • Averaging Time UV(s): 1.3
    • Eluent A Inlet: A2
    • Start Conc B (%): 0
    • Column Valve: Column_Position: Position 4
    • Column Equilibration: Equilibrate w/ (CV): 1.0
    • Aut_PressureFlow_Reg:
      • System Pump: Normal
      • System Press Level (MPa): 0
      • System Min Flow (mL/min): 0
    • Flowthrough Fractionation:
      • Tube Size (mm):18
      • Fraction Size (mL): 5
      • Flowthrough Fract Start at: First Tube
    • Sample Injection: Empty Loop w/ (mL): 2.0
    • Wash out Unbound Sample: Wash column w/ (CV): 10
    • Fractionation
      • Tube Type Eluate Fraction (mm): 18
      • Eluate Frac Size (mL): 1.0
      • Eluate Fract Start at: Next Tube
      • Tube Type Peak Fraction (mm): 18
      • Peak Frac Size (mL): 0
      • Peak Fract Start at: First Tube
    • Linear Gradient:
      • Target Conc B (%): 100
      • Length of Gradient (CV): 5
    • Gradient Delay: Gradient Delay (mL): 0
    • Clean after Elution: Clean w/ (CV): 2

CAjoFmethodHis1mL

  • Note: same as CAjoFmethodHis except:
    • Main:Column: HisTrap_FF_1mL
    • Fractionation:Eluate Frac Size (mL): 0.25
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