Neutral Red for gHV68 (DW)
- Neutral Red
- Whatman filter paper
- Add 25g neutral red to 2 liters water.
- Incubate o/n at 56C protected from light.
- Filter through Whatman filter paper.
- Aliquot into small bottles and wrap in tin foil.
- Store stock at RT.
- Dilute 3 ml stock solution per 100 ml PBS.
- Apply to methylcellulose.
- 242.5ml PBS + 7.5ml Stock Neutral Red = 250ml Working Stock Neutral Red
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