Nick Rohacz: Week 4

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Terminology

  1. The Michealis-Menten model of enzyme kinetics is a model used to measure the concentrations of enzymes, product, and substrate using rate laws and the model enzyme + substrate ↔ enzyme/substrate → enzyme + product.
  2. The experiment required for the Lineweaver-Burk approach to estimating parameters for Michealis-Menten produces a linear form of Michealis Menten where K is the slope/intercept and Vmax is 1/intercept.
  3. A Chemostat Reactor is a reactor in which the conditions are easily controlled by keeping homeostasis througha static equilibrium, thus allowing controlled growth of the organism.
  4. Exponential Growth is when the output of a model is not linear with the input, but rather the output far exceeds the amount input. Similar to the graph y=ex.

Applying Michealis-Menten Models

Image:NAR_CAT.jpg Image:NAR_CAT2.jpg Image:NAR_CAT3.jpg

Estimating Michealis-Menten Parameters

Slope = 859.23 Intercept = 31.695 Vmax = 1/intercept = .03155 K = slope/intercept = 27.109

Nicholas A. Rohacz 23:24, 6 February 2011 (EST)

Class Assignments

Nick Rohacz: Week 2 Nick Rohacz: Week 6 Nick Rohacz: Week 11
Nick Rohacz: Week 3 Nick Rohacz: Week 7 Nick Rohacz: Week 12
Nick Rohacz: Week 4 Nick Rohacz: Week 8 Nick Rohacz: Week 13
Nick Rohacz: Week 5 Nick Rohacz: Week 9 Nick Rohacz: Week 14

Summer Research

Computational Journal

Class Journals

Nick Rohacz: Week 1 Nick Rohacz: Week 5 Nick Rohacz: Week 9 Nick Rohacz: Week 13
Nick Rohacz: Week 2 Nick Rohacz: Week 6 Nick Rohacz: Week 10 Nick Rohacz: Week 14
Nick Rohacz: Week 3 Nick Rohacz: Week 7 Nick Rohacz: Week 11
Nick Rohacz: Week 4 Nick Rohacz: Week 8 Nick Rohacz: Week 12


Individual Work

Nitrogen Metabolism

Useful links

Personal tools