|Lecture 1: A discussion about Biophysics in General, and Biophysics in the UNM Physics Department. Random Walks, Stokes-Einstein relation, time scales of diffusion in cell membranes and cytoplasm. (Nelson Chapter 4)
|Lecture 2: Basic structure and function of Eukaryotic cells. (Nelson Chapter 2) Diffusion through channels and membranes. (Nelson Chapter 4) Lecture 3: Quiz on basic biology terminology. Friction in fluids and low Reynolds number. (Nelson Chapter 5).
|No Classes: Biophysical Society Meeting Feb 2-6. Classes will be rescheduled at a later date.
|Lecture 4:: Basic chemistry in Biology, the Navier-Stokes equation, Reynolds number and low viscosity environments (Nelson Chapter 5).
Lecture 5:: Basic chemistry in Biology II, disorder, maximum entropy lead to the Maxwell-Boltzmann distribution. Energy, enthalpy and free energies (Nelson Chapter 6).
|Lecture 6:: Rate constants, chemical equilibrium, Arrhenius equation.
Lecture 7:: Quiz on basic biochemistry (postponed from Monday). Entropic forces.
|Lecture 8:: Chemical Potential, Osmosis, Rate equations, Scatchard Plots
Lecture 9:: Gels, Freely jointed chains, 1D cooperative chain model.
|Lecture 10:: Worm-Like Chain Model
Lecture 11:: Buffers, Fluorescence Correlation Spectroscopy, Basic Microscope Optics.
|Lecture 12:: Point Spread Function Calculations.
Lecture 13:: MATLAB PSF demo, Photodiode, Avalanche Photodiodes.
|Lecture 14:: PMTs, CCDs, Exam Overview
Reveiw Session: Review for Exam/ Q&A problems session. 3:00pm, meet outside rm 5.
March 26: Midterm Exam.
March 31,April 2
|Lecture 15,16:: Parameter Estimation, Cramer-Rao Lower Bound, single fluorophore localization accuracy.
|Lecture 17:: Beer-Lambert Law, Protein and Nucleic Acid concentration measurements, Phospholipid nomenclature, Laser Classes.
Lab 1: Single Particle Tracking of Quantum Dot Labeled lipids in a DOPC bilayer.
|Lecture 18,19:: Single Particle Tracking Analysis, lipid bilayer elasticity, Optical trap calibration for optical tweezers.
|Lecture 18: Molecular Motors. April 23: Quiz 3- click here for topics