PIPES

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piperazine-N,N’-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757

A Good buffer [1][2][3].

  • pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
  • usable buffering range of 6.1 to 7.5
  • molecular weight 302.4
  • ΔpKa/ΔT = -.0085

Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. Hydrogen ion buffers for biological research. Biochemistry 1966 Feb; 5(2) 467-77. pmid:5942950. PubMed HubMed PubGet [Good66]
  2. Good NE and Izawa S. Hydrogen ion buffers. Methods Enzymol 1972; 24 53-68. pmid:4206745. PubMed HubMed PubGet [Good74]
  3. Blanchard JS. Buffers for enzymes. Methods Enzymol 1984; 104 404-14. pmid:6717292. PubMed HubMed PubGet [Blanchard84]
All Medline abstracts: PubMed HubMed and
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