Primers gano

ordered received Name Sequence (5'->3') Comments EXAMPLE --- HA1 CTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATG Homology arm at insertion site in GFP (forward strand)

Name Sequence Comments

• * pHg_LB_For(sequencing) TGGTGGCAGGATATATTGTGGTGTAAACA This is to amplify the T-DNA region in pHg (or any pCAMBIA-1300 based vector)
• * pHg_RB_Rev(sequencing) ccgttattctaataaacgctcttttctcttaggtttac This is to amplify the T-DNA region in pHg (or any pCAMBIA-1300 based vector)

• * pSILBAgamma_For(sequencing) ggatcctctagaaagaaggattacctctaaacaag For amplifying silencing cassette region from pSILBAgamma
• * pSILBAgamma_Rev(sequencing) gaattcagaggtccgcaagtagattgaaag

• * pHg_L.bicolor_internalSeq_F aaagcatcagctcatcgagagcc

• * pSILBA_For_seq2 tacgccaagcgcgcaattaac
• * pSILBA_Rev_seq2 caaggcgattaagttgggtaacgc

• psdhB_H2L_for aatgaatgaccaacaagaccgt This is to amplify the H->L mutation in the psdhB_GL_ plasmid
• psdhB_H2L_rev acacctacctctccgccat This is to amplify the H->L mutation in the psdhB_GL_ plasmid

• * pHg_rev_sdhBp CTTGAGATATGAAAAAGCCTGAACTCACC no overhang
• * pHg_for_sdhBp TTCTTCAGCTTGATATCGAATTCCTGC w/ 20bp OH for Gibson w/ sdhBp

• * pHg_for_HyrgoR(Gano) atctcaagccatggcgataagcttg no OH
• * pHg_rev_HygroR(gano) agtagatgccgaccggaagc no OH

n/a n/a pHg_rev_GPDp cttgagatatgaaaaagcctgaactcacc no OH, can use pHg_rev_sdhBp n/a n/a pHg_for_GPDp TTCTTCAGCTTGATATCGAATTCCTGCAG no OH, can use pHg_for_sdhBp

• * pHg_forMid_HygroR(gano) AGCCATGACCCAGTCACGTAGC
• * pHg_revMid_HygroR(gano) cgcatagttaagccagtatacactccg

• * pHg_rev_hphSeq cgtctcgaatgttctcggtgtttagg
• * pHg_for_hphSeq CACATGAGCGAAACCCTATAGGAACC

• * pHg_for_SeqPrmtr AAACTCCCCAATGTCAAGCACTTCC
• * pHg_rev_SeqPrmtr tcgtgccagctgcattaatgaatc

06/2020

• * F_G3PDp_105971SS1 AGAGGCGTCGTCAACG
• * R_rfp_G3PDp_10597SS1 ACGTCCTCGCTGCTGGCCATCCGTTAATTCCGACTTTGACCTAG
• * F_rfp_G3PDt_10597SS1 ACTCCGCCTCCGTTGACCGCGTAGAGGCAGATTGCAAGGTCTTG
• * R_G3PDt_10597SS1 AATCGCGAGCTCACTAATCACCC
• * R_RFP_ohG3PDt ACCTTGCAATCTGCCTCTACGCGGTCAACGGAGGC
• * F_RFP_G3PDp GTCAAAGTCGGAATTAACGGATGGCCAGCAGCGAGG

• * F_G3PDp_OHpHg GACGTTTTTAATGTACTGAAAGAGGCGTCGTCAACG
• * R_G3PDp_OH_pHg_mRFP GCCGACCGATTCGGCGTTAAAATCGCGAGCTCACTAATCACCC

• * F_pHg_OH_G3PDp_mRFP TGATTAGTGAGCTCGCGATTTTAACGCCGAATCGGTC
• * R_pHg_OH_G3PDp_mRFP AGGGCGTTGACGACGCCTCTTTCAGTACATTAAAAACGTCCGC

• pshdB_1_midFor caggacgaaccgtttttcattaccg
• psdhB_1_midRev ctccgcctcgatctcttcgg

• * seq_midbkbn_0001 gcttaactatgcggcatcagagc
• * seq_pro_0001 gactgatgggctgcctgtatcg
• * seq_RFP_0001 GCGACGGAGCTATTAGGACATGG
• * seq_term_0001 GTGCGTGTAAAACTGAAAGACCGTG
• * seq_bk2_0001 GCTTCGGGCCATATCAACGC

• psdhB_seqFor_sdhBp cagatcggtaccagccaaatgc

pHg(Ecoli)_G3PDp(GanoLuc)

• * pHg_Rev_G3PDpGL GTCAAAGTCGGAATTAACGGATGAAAAAGCCTGAACTCACCGC
• * Rev_G3PDp_pHgLB GTGAGTTCAGGCTTTTTCATCCGTTAATTCCGACTTTGACCTAGAAGTAC

• * pHg_mRev_2020 GCAGCGACCGAAGGGTAGG
• * pHg_mFor_2020 CAGCCGAAGAGCTGCAAAAAGC

• * For_G3PDpGL_pHgLB TTCGATATCAAGCTGAAGAAAGAGGCGTCGTCAACGCC
• * pHg_For_G3PDpGL AGGGCGTTGACGACGCCTCTTTCTTCAGCTTGATATCGAATTCCTGCAG

pHg_GL

• * F_pHgGL_G3PDt TGATTAGTGAGCTCGCGATTTTCTTCAGCTTGATATCGAATTCCTGC
• * R_G3PDt_pHgGL TTCGATATCAAGCTGAAGAAAATCGCGAGCTCACTAATCACCC
• *
• * F_G3PDt_hph GTCCCCGGGCTAAAAAGTGAGTAGAGGCAGATTGCAAGGTC
• * R_hph_G3PDt ACCTTGCAATCTGCCTCTACTCACTTTTTAGCCCGGGGACG
• *
• * F_hph_G3PDp GTCAAAGTCGGAATTAACGGATGAAGAAGCCGGAGCTTAC
• * R_G3PDp_hph GTAAGCTCCGGCTTCTTCATCCGTTAATTCCGACTTTGACCTAGAAG
• *
• * F_G3PDp_pHgGL GTACTGAATTAACGCCGAATAGAGGCGTCGTCAACG
• * R_pHgGL_G3PDp AGGGCGTTGACGACGCCTCTATTCGGCGTTAATTCAGTACATTAAAAAC

pcbx_mRFP

• * F_G3PDp_pCbxOH CAGCTATGACCATGATTACGAGAGGCGTCGTCAACG
• * R_pCbx_G3PDpOH AGGGCGTTGACGACGCCTCTCGTAATCATGGTCATAGCTGTTTCCTGTG

• * R_G3PDt_pCbxOH TGATCCAAGCTCAAGCTGCTAATCGCGAGCTCACTAATCACCC
• * F_pCbx_G3PDtOH TGATTAGTGAGCTCGCGATTAGCAGCTTGAGCTTGGATCAGATTG

• * hph_pHg_seq aggcaggtcttgcaacgtg hs

• * Rseq_pHg CACATACAAATGGACGAACGGATAAACC rs

• cbxFor

• cbxRev

• * R_g3pdt_popen CCACTTCTTCCCCGATAACGAATCGCGAGCTCACTAATCACCC

• * F_g3pdp_popen ACCGATATGGCCAGTGTGCCAGAGGCGTCGTCAACG

• * R_popen_g3pdp AGGGCGTTGACGACGCCTCTGGCACACTGGCCATATCGG
• * popen_midFor CAACTTTATCCGCCTCCATCCAGTC

• * F_popen_g3pdt TGATTAGTGAGCTCGCGATTCGTTATCGGGGAAGAAGTGGCTG
• * popen_midRev CTGGCGAACTACTTACTCTAGCTTCC

• * seqRev_G3PDt_2 GTATGTACCTCCGGTTTGCGTGATC

pHygR

• * r_pHygR_G3PDt TGATTAGTGAGCTCGCGATTATTCGGCGTTAATTCAGTACATTAAAAAC
• * f_pHygR_g3pdp AGGGCGTTGACGACGCCTCTTTCTTCAGCTTGATATCGAATTCCTGC

• * f_g3pdp_pHygR TTCGATATCAAGCTGAAGAAAGAGGCGTCGTCAACGCC
• * r_g3pdt_pHygR GTACTGAATTAACGCCGAATAATCGCGAGCTCACTAATCACCC

pCbxR(RP)_fuGFP

• * f_fuGFP_g3pdp GTCAAAGTCGGAATTAACGGATGGTCAGCAGCGGGGAGG
• * r_fugfp_g3pdt ACCTTGCAATCTGCCTCTACTCAGGACTTGTAAAGCTCGTCCATGC

• * r_g3pdp_fugfp TCCTCCCCGCTGCTGACCATCCGTTAATTCCGACTTTGACCTAGAAG
• * f_g3pdt_fugfp ACGAGCTTTACAAGTCCTGAGTAGAGGCAGATTGCAAGGTC

pOpen_Myco_HygR

• * r_popen2_g3pdp AGGGCGTTGACGACGCCTCTGCGATGGCGAAGACTACTCCC
• * f_popen2_g3pdt TGATTAGTGAGCTCGCGATTGCGATGTTGAAGACCACGCTGAG

• * f_g3pdp2_popen GGAGTAGTCTTCGCCATCGCAGAGGCGTCGTCAACGC
• * r_g3pdt2_popen AGCGTGGTCTTCAACATCGCAATCGCGAGCTCACTAATCACCC

pOpen_Myco_fuGFP

• * mfor_popen GCTCATCATTGGAAAACGTTCTTCGG
• * mrev_popen GTTACATCGAACTGGATCTCAACAGCG

pOpen_Myco_NanoLuc

• * r_g3pdp_nanoluc TCTTCGAGTGTGAAGACCATCCGTTAATTCCGACTTTGACCTAGAAG
• * f_g3pdt_nanoluc GCGAACGCATTCTGGCGTAAGTAGAGGCAGATTGCAAGGTCTTG

• * f_nanoluc_g3pdp GTCAAAGTCGGAATTAACGGATGGTCTTCACACTCGAAGATTTCG
• * r_nanoluc_g3pdt ACCTTGCAATCTGCCTCTACTTACGCCAGAATGCGTTCGC

• F_pOM_HygR ggctacggtctcccttcttgagaagaactagcataaccccttggggcctc 100nm STD
• R_pOM_HygR ggctacggtctcattgcgtttgtcctcagcggctccgacgattgacac 100nm STD

• F_TEF1t ggctacggtctcagcaaagaaacccccgggagtag 100nm STD
• R_TEF1t ggctacggtctctagtcctgaggcccactgagcatgatctgatc 100nm STD

• F_fuGFP_pOMHygR ggctacggtctctgacttgtaaagctcgtccatgcc 100nm STD
• R_fuGFP_pOMHygR ggctacggtctcttggtcagcagcggggaggatat 100nm STD

• F_TEF1p ggctacggtctctaccatcatggtcctcagggctgagatc 100nm STD
• R_TEF1p ggctacggtctccgaagcacttccgaatttgcattatcag 100nm STD